Guard cell redox proteomics reveals a role of lipid transfer protein in plant defense

Redox-based post-translational modifications (PTMs) involving protein cysteine residues as redox sensors are important to various physiological processes. However, little is known about redox-sensitive proteins in guard cells and their functions stomatal immunity. In this study, we applied an integrative labeling method cysTMTRAQ, identified cell that were altered by thiol PTMs response a bacterial flagellin peptide flg22. total, eight, seven 20 potential redox-responsive treated with flg22 for 15, 30 60 min, respectively. The fall into several functional groups including photosynthesis, lipid binding, oxidation-reduction, defense. Among the proteins, transfer (LTP)-II was confirmed be involved plant resistance Pseudomonas syringe pv. tomato DC3000. This study not only creates inventory of signal transduction cells, but also highlights biological relevance defense against pathogens. Protein play roles many proteomics has rarely been studied single cell-types. isobaric tandem mass tag-based technology discover corresponding Many related identified. Using reverse genetics biochemical analyses, functionally characterized utility discovering new functions. Furthermore, detailed characterization demonstrates pathogen